Construction of a regulatable cancer-specific adenoviral expression system using human telomerase reverse transcriptase gene promoter.
نویسندگان
چکیده
We constructed a novel cancer-specific regulatable adenoviral expression system comprising two vectors: one expressing rtTA, a reverse tetracycline transactivator regulated by the human telomerase reverse transcriptase (hTERT) gene promoter, the other expressing the target gene regulated by the tetracycline response element (TRE). rtTA transactivates target gene expression in the presence of doxycycline. Using these vectors, we constructed an adenoviral expression system, the Tel-On system, which enables highly efficient target gene transduction. This system enabled efficient and regulatable cancer-specific gene expression, and can be used in targeted cancer gene therapy.
منابع مشابه
Tumor-specific transgene expression from the human telomerase reverse transcriptase promoter enables targeting of the therapeutic effects of the Bax gene to cancers.
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ورودعنوان ژورنال:
- Bioscience, biotechnology, and biochemistry
دوره 72 6 شماره
صفحات -
تاریخ انتشار 2008